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Expression patterns of ESC compared to TRF-O3 feeder cells in 2i and YPAC medium. Transcription levels of pluripotency and differentiation markers were compared in ES21 from passage 7 and passage 15 cultured of TRF-O3 as feeder cells in 2i-LIF and YPAC medium performing RT-PCR. + positive control: pooled cDNAs from pluripotent rat embryonic germ cell lines (Northrup et al. 2011). GAPDH was used as endogenous control.
