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Differentiation of ES21 cells and primary ESC in YPAC or 4i medium on TRF-O3 cells. a-c. Differentiation of ES21 cells started between day 1 and day 3 after cultivation in YPAC medium. d-f. In contrast the ESC remained undifferentiated when synchronously cultured in 2i-LIF medium. Freshly isolated ESC also underwent differentiation in g. YPAC medium or h. 4i medium, phase contrast (10×), scale bar is 100 μm.
