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. 2014 Oct 6;7:462. doi: 10.1186/s13071-014-0462-1

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© Voronin et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Visualisation of PAL and VirB6 in whole worm mounts of B. malayi . A, B, Wolbachia (small red foci, bacteria DNA stained by propidium iodide, large red spot, nematode nuclei, see Methods) and PAL (green, secondary antibody labeled by FITC) in lateral cord of filarial nematode, C, merged A and B. D, E, VirB6 (green) and Wolbachia (small red foci) in B. malayi (wBm). F, merged D and E. G, H, negative control – Wolbachia-free B. malayi treated with antibiotic (lack of PAL reactivity shown as representation of both antibodies). I, merged G and H.