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Western blot on protein extract. CaSR protein possesses different levels of maturation in terms of post-translational modifications: with respect to the WT, the 972 M mutant showed underexpressed the fully mature glycosylated form of 160 kDa, while the other two bands (the immature not-glycosylated 140 kDa form and the dimers band of 280 kDa) showed a normal pattern.
