Figure 4.

CaSR signaling activity measured as the ratio between the level of phosphorylated and total p42/44 proteins. a: compared with the WT for both the Ca²⁺ concentrations, the 972 M variant induced a lower level of phosphorylation, similarly to the activity mediated by the inactivating control (F351V). Values were normalized with respect to the WT at 10 mM. Data are reported as mean ± SEM of three replicate experiments. * = p <0.05 compared with the WT; b: a representative western blot was shown.