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. 2014 Oct 4;13:393. doi: 10.1186/1475-2875-13-393

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© Tran et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Correlation between parasite densities determined by light microscopy and calculated by nested qPCR. Calculated parasite densities from nested qPCR were plotted against parasite densities determined by light microscopy for symptomatic infections (red circles), asymptomatic infections with positive blood smears (black circles), and asymptomatic infections with negative blood smears (unfilled circles). Parasite densities calculated by nested PCR strongly correlated with both asymptomatic (Pearson’s r = 0.58, 95% CI [0.29 to 0.77], P < 0.001) and symptomatic (Pearson’s r 0.70, 95% CI [0.53 to 0.81], P < 0.0001) P. falciparum infections. The dashed line represents the limit of detection for blood-smear microscopy (~40 parasites/μl).