Table 2.
Task-Relevant Sensory Behaviors and Osmotactic Response to an Attractive Odor
| Benzaldehydea
|
Octanolb
|
Electroshockc
|
|||||
|---|---|---|---|---|---|---|---|
| Genotype | 1x | 0.1x | 1x | 0.1x | 90V | 45V | Geraniold |
| c492 | 73.24 ± 2.31 | 61.59 ± 4.18 | 78.91 ± 4.54 | 59.86 ± 5.07 | 76.31 ± 3.61 | 56.41 ± 6.44 | 68.05 ± 4.75 |
| c772 | 87.98 ± 3.33 | 66.57 ± 3.79 | 86.91 ± 2.21 | 71.23 ± 4.54 | 77.09 ± 4.02 | 60.17 ± 5.06 | 62.61 ± 7.57 |
| UAS-btau/+ | 80.13 ± 2.39 | 59.22 ± 2.43 | 83.96 ± 2.33 | 59.04 ± 4.55 | 69.05 ± 5.09 | 58.51 ± 4.01 | 78.94 ± 4.48 |
| c492/+; UAS-btau/+ | 86.31 ± 4.33 | 73.83 ± 2.16 | 87.35 ± 4.87 | 69.97 ± 4.41 | 82.91 ± 4.09 | 58.26 ± 3.28 | 67.87 ± 4.35 |
| c772/+; UAS-btau/+ | 76.03 ± 5.26 | 67.55 ± 2.73 | 82.64 ± 2.78 | 74.09 ± 6.73 | 70.24 ± 5.29 | 49.11 ± 8.41 | 70.19 ± 4.13 |
| UAS-htauwt1 | 82.89 ± 2.86 | 68.73 ± 3.45 | 79.78 ± 3.27 | 62.37 ± 3.87 | 74.59 ± 4.68 | 56.78 ± 5.27 | 66.72 ± 4.62 |
| c492/+; UAS-htauwt1/+ | 81.26 ± 3.67 | 64.58 ± 2.96 | 82.47 ± 3.98 | 67.79 ± 4.39 | 76.46 ± 4.89 | 54.89 ± 4.78 | 71.87 ± 4.64 |
| c772/+; UAS-htauwt1/+ | 78.76 ± 2.97 | 67.81 ± 3.66 | 81.78 ± 4.27 | 70.91 ± 2.98 | 78.68 ± 3.95 | 53.97 ± 5.17 | 68.72 ± 4.49 |
| UAS-dtau1/+ | 81.66 ± 2.78 | 67.12 ± 3.26 | 85.48 ± 3.08 | 66.27 ± 3.74 | 76.82 ± 2.99 | 49.93 ± 5.34 | 64.48 ± 4.32 |
| UAS-dtau1/+ at 29°C | 80.03 ± 3.10 | 63.93 ± 2.93 | 86.69 ± 3.34 | 61.94 ± 3.92 | 73.27 ± 3.46 | 52.78 ± 4.97 | 68.72 ± 4.49 |
| UAS-dtau4/+ | 82.14 ± 2.37 | 63.48 ± 3.57 | 80.96 ± 2.87 | 64.36 ± 4.28 | 74.08 ± 4.29 | 56.82 ± 4.39 | 67.24 ± 5.27 |
| c492/+; UAS-dtau1/+ | 77.73 ± 3.76 | 65.28 ± 2.84 | 80.46 ± 3.24 | 66.15 ± 3.94 | 79.12 ± 4.53 | 51.32 ± 4.88 | 69.47 ± 4.78 |
| c492/+; UAS-dtau1/+ at 29°C | 84.23 ± 4.24 | 71.17 ± 4.21 | 84.25 ± 2.82 | 60.82 ± 4.08 | 75.14 ± 4.28 | 52.36 ± 4.67 | 66.86 ± 4.38 |
| c772/+; UAS-dtau1/+ | 78.76 ± 2.97 | 64.59 ± 3.49 | 82.47 ± 3.54 | 65.38 ± 4.73 | 75.27 ± 3.77 | 56.23 ± 5.43 | 65.84 ± 5.49 |
| c772/+; UAS-dtau1/+ at 29°C | 81.57 ± 3.17 | 69.22 ± 3.62 | 86.18 ± 3.21 | 68.17 ± 4.46 | 71.89 ± 4.95 | 55.62 ± 4.71 | 67.48 ± 4.72 |
| c492/+; UAS-dtau4/+ | 84.76 ± 2.72 | 70.88 ± 4.04 | 83.85 ± 2.73 | 63.36 ± 3.92 | 76.36 ± 4.16 | 52.88 ± 4.64 | 63.87 ± 4.97 |
| c772/+; UAS-dtau4/+ | 80.69 ± 3.18 | 66.47 ± 3.29 | 82.94 ± 2.58 | 67.11 ± 4.24 | 74.83 ± 3.78 | 57.74 ± 4.89 | 65.47 ± 4.28 |
Avoidance of benzaldehyde at the concentration used for conditioning (1×, column 2), or a 10-fold dilution (0.1 ×, column 3; n ≥ 7). Although the effect of benzaldehyde dilution was significant (F(1, 271) = 9.075, p < 0.005), ANOVA did not reveal differences among strains at either dilution.
Avoidance of octanol as used for conditioning (1 ×, column 4), or a 10-fold dilution (0.1 ×, column 5; n ≥ 7). Significant effects were revealed for odorant dilution (F(1, 267) = 11.624, p < 0.005) and marginally among strains for 0.1 × octanol avoidance (F1, 134) = 3.987, p < 0.05). Subsequent pairwise comparisons revealed differences among all strains and c772/+, c772/+; UAS-btau/+; heterozygotes, which is unlikely to have contributed to defects in associative behaviors (Fig. 4) because the c772 control is normal in that assay.
Avoidance of electrified grids kept at 90 V (column 6) or 45 V (column 7; n ≥ 7). Two-way ANOVA revealed significant effects of stimulus strength (F1, 262) = 11.873, p<0.005), but among strains either at 90 or 45 V.
Osmotaxis and navigation toward an attractive odor (column 8). There was no statistical difference in attraction toward 0.05% geraniol and entrance into the olfactory traps (n ≥ 12).