Table III.
Sorbitol (8–12% w/v), MgCl2 (5–12% w/v) and MSG (5–12% w/v) Tested in a Central Composite Circumscribed Design. DU Recoveries were Determined per Serotype Directly After Lyophilization and After Accelerated Stability Testing (one week at 45°C)
| Sorbitol | MgCl2 | MSG | DU recovery (%) | DU recovery (%) | |||||
|---|---|---|---|---|---|---|---|---|---|
| Directly after lyophilization | Stability 45°C | ||||||||
| T1 | T2 | T3 | T1 | T2 | T3 | ||||
| O1 | 8% | 5% | 5% | 98.6 | 96.5 | 85.7 | 91.9 | 97.0 | 55.2 |
| O2 | 12% | 5% | 5% | 88.9 | 88.7 | 65.2 | 87.6 | 91.6 | 73.7 |
| O3 | 8% | 12% | 5% | 76.0 | 82.4 | 33.6 | 62.5 | 49.2 | 12.2 |
| O4 | 12% | 12% | 5% | 89.4 | 91.7 | 59.1 | 95.8 | 90.8 | 48.2 |
| O5 | 8% | 5% | 12% | 90.6 | 81.9 | 70.7 | 86.0 | 81.4 | 57.5 |
| O6 | 12% | 5% | 12% | 79.3 | 82.5 | 62.1 | 80.2 | 77.0 | 39.4 |
| O7 | 8% | 12% | 12% | 84.9 | 86.7 | 64.6 | 92.0 | 84.6 | 63.5 |
| O8 | 12% | 12% | 12% | 87.9 | 82.5 | 73.0 | 90.3 | 86.7 | 69.8 |
| O9 | 6.6% | 8.5% | 8.5% | 90.0 | 99.3 | 84.8 | 93.3 | 90.6 | 73.0 |
| O10 | 13.4% | 8.5% | 8.5% | 86.2 | 85.3 | 67.4 | 86.1 | 85.3 | 69.3 |
| O11 | 10% | 2.6% | 8.5% | 77.5 | 72.4 | 47.4 | 74.4 | 60.7 | 29.9 |
| O12 | 10% | 14.4% | 8.5% | 89.1 | 84.6 | 63.6 | 91.7 | 72.7 | 40.0 |
| O13 | 10% | 8.5% | 2.6% | 98.1 | 85.2 | 49.2 | 93.0 | 83.3 | 42.2 |
| O14 | 10% | 8.5% | 14.4% | 84.7 | 70.3 | 66.3 | 74.8 | 81.2 | 35.5 |
| O15 | 10% | 8.5% | 8.5% | 86.6 | 88.4 | 68.2 | 90.1 | 91.1 | 67.3 |
| O16 | 10% | 8.5% | 8.5% | 92.2 | 90.8 | 75.1 | 93.5 | 89.1 | 69.2 |
| O17 | 10% | 8.5% | 8.5% | 96.0 | 98.3 | 76.7 | 91.7 | 94.0 | 57.0 |