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. 2014 Sep 12;3(10):924–936. doi: 10.1242/bio.20148185

Fig. 3. p85β modulates adhesion structures by increasing central adhesions.

Fig. 3.

(A,B) WT, p85α−/− or p85β−/− MEF (A,C) or NIH3T3 cells transfected with p85α or p85β (B) were stained with anti-paxillin or -vinculin Ab. Images show the first confocal section from the adhesion plane. Transfected cells were identified by anti-p85β Ab (K1123) or -pan-p85 Ab (for p85α) (insets) and are indicated by an arrowhead. Graphs show the number of peripheral or central adhesions per cell (mean ± s.d.; n  =  25). Focal contacts and focal adhesions near the cell membrane were considered peripheral; rounded adhesions at the cell center (not in contact with the membrane) were considered central adhesions. (C) MEF as in (A) were stained using phalloidin-TRITC. Arrows indicate F-actin dots at the cell first z-section, in contact with the matrix. Bar  =  12 µm. *** P<0.001, ** P<0.01, *-P<0.05; Student's t-test.