Fig. 6.

Downregulation of genes encoding chromatin modifiers and HDAC1 regulation by FGF signalling. (A) Transcripts for key chromatin-modifying genes SMARCA2, JARID2 and HDAC1 are downregulated as neural differentiation begins, whereas expression of EP300, which counters the activity of HDAC1, increases (arrowheads indicate the most recently formed somite; black dashed lines indicate the position of transverse sections beneath each embryo). (B) FGF-presenting beads transplanted into the stem zone maintain local ectopic HDAC1 transcription (white arrow). (C) Beads presenting the FGFR inhibitor PD173074 locally repress HDAC1 transcription in the stem zone (white arrow). Scale bars: 100 µm.