(A) Total spleen CD4 cells from TLR4−/−, IL-10−/−, TLR4−/−IL-10−/− [double-knockout (dKO)], and WT mice were analyzed for IFN-γ and IL-17 production. (B) IFN-γ and IL-17 expression from total CD4+ T cells in the spleen and intestine. Bar charts reflect mean ± sem. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with WT mice. Data are representative of three or more experiments of two or more mice/group with similar results. FACS plots are gated upon live CD4+ cells.