Figure 3. Helios+Nrp1+ Treg and Helios−Nrp1− Tregs differentially express IFN-γ in the spleen.
(A) Tregs (CD4+Foxp3+) were separated into Helios+Nrp1+ and Helios−Nrp1− Tregs, and analyzed for IFN-γ and IL-17 expression from the spleen of TLR4−/−, IL-10−/−, TLR4−/−IL-10−/−, and WT mice. (B) Helios+Nrp1+ Tregs [double-positive (DP) tTreg] and Helios−Nrp1− Tregs [double-negative (DN) iTreg] from the spleen of TLR4−/− IL-10−/− and WT mice were analyzed for IFN-γ and IL-17 production. Bar charts are calculated based on total double-positive tTreg or double-negative iTreg and reflect mean ± sem. *P < 0.05, and **P < 0.01. Data are representative of three or more experiments of two or more mice/group. FACS plots are gated upon live CD4+Foxp3+ cells.