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(A) Spleen CD4 cells from TLR4−/−, IL-10−/−, TLR4−/−IL-10−/−, and WT mice were counted for Foxp3 expression and IFN-γ and IL-17 in Tregs. (B) Tregs (CD4+Foxp3+) were analyzed for Helios and Nrp expression. DP tTreg, Helios+Nrp1+ Tregs; DN iTreg, Helios−Nrp1− Tregs. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with WT B6 mice. Data are representative of three or more experiments of two or more mice/group.
