Figure 5. Intestinal Helios⁺ Nrp1⁺ T_regs and Helios⁻Nrp1⁻ T_regs differentially produce IFN-γ and IL-17.

(A) T_reg (CD4⁺Foxp3⁺) cells were separated into Helios⁺Nrp1⁺ and Helios⁻Nrp1⁻ T_regs and analyzed for IFN-γ and IL-17 expression from the intestines of TLR4^−/−, IL-10^−/−, TLR4^−/−IL-10^−/−, and WT mice. (B) Helios⁺Nrp1⁺ T_regs (DP tT_reg) and Helios⁻Nrp1⁻ T_regs (DN iT_reg) from the intestines of TLR4^−/−IL-10^−/− and WT mice were analyzed for IFN-γ and IL-17 production. Bar charts are calculated based on total double-positive tT_reg or double-negative iT_reg and reflect mean ± sem. *P < 0.05, **P < 0.01, and ***P < 0.001. Data are representative of three or more experiments of two or more mice/group. FACS plots are gated upon live CD4⁺Foxp3⁺ cells.