Figure 7. Expansion and acquisition of proinflammatory cytokine production by T_regs occur in the intestine.

CBir1 CD4 T cells (0.25×10⁶) were i.v. transferred into RAG2^−/− mice. Recipient mice were gavaged with PBS or FTY720 every 2 days. (A) Four weeks post-T cell transfer, the histopathology of intestines was determined. Images are representative of two experiments of four mice/group with similar results. (B) Pathological scores are shown. (C) T_regs were isolated from the spleens and intestinal LP of CBir1 CD4 T cell recipient mice and analyzed for Foxp3, IFN-γ, and IL-17 production by flow cytometry. FACS plots are gated on total live CD4⁺ T cells. Data are representative of two experiments of four mice/group with similar results.