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. 2014 Nov;96(5):703–712. doi: 10.1189/jlb.3RU0214-110R

Figure 3. LBs, cytoplasmic organelles not delimited by a bilayer membrane, are formed rapidly in response to leukocyte activation.

Figure 3.

(A) LBs are delimited by a monolayer of phospholipids (yellow box, arrowheads), differing from the classical, structural organization (phospholipid bilayer membrane) of cytoplasmic granules (red box, arrowheads), vesicles, and plasma membranes (blue box, arrowheads). (B) In TNF-α-activated eosinophils, a large number of LBs with different sizes are seen in the cytoplasm as dark, osmium-stained organelles. Arrows indicate small LBs. (C) Stimulation of eosinophils with inflammatory chemokines and cytokines induces significant formations (*P≤0.05) of LBs compared with NS eosinophils. Naturally activated eosinophils from HES patients also showed increased numbers of LBs. Cells were isolated from the peripheral blood of healthy donors or an HES donor, stimulated or not with CCL11/eotaxin-1, CCL5/RANTES, TNF-α, or IFN-γ, and processed for TEM using conventional (A) or reduced (B) osmium [11]. Original scale bars, 500 nm (A) and 900 nm (B). (B and C) Reprinted from ref. [11], with permission.