Figure 4. CD47 triggering in differentiating Cnt MO results in decreased CD1a⁺MDDC differentiation and development of a CD1a⁺DC population with down-regulated costimulatory receptors and decreased antigen-processing capability.

MO from healthy volunteers were differentiated to MDDC in the presence of CD47 triggering by αCD47 antibody (CD47 DC) or 4N1K peptide (4N1K DC). MDDCs similarly differentiated in the presence of an isotype Cnt antibody, or a nonspecific peptide served as Cnt (Cnt DC). Six day-differentiated MDDCs were assessed for CD1a (A) and CD14 (B) expression by flow cytometry. (C) CD1a⁺MDDC antigen processing capacity was assessed by gating on CD1a⁺MDDCs and measuring DQ-OVA processing with flow cytometry. (D) CD1a⁺MDDC expression of CD86 was assessed by flow cytometry. For all graphs, ^#P < 0.05 and *P < 0.01 by Wilcoxon matched pairs test, n = 7 Cnt DC, 4 CD47 DC, and 3 4N1K DC. Data are represented as mean ± sem.