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. 2014 Nov;96(5):907–915. doi: 10.1189/jlb.3A0314-152R

Figure 2. PDE2A and iNOS protein expression by Western immunoblot in MH-S macrophages that were first infected with increasing Ad MOI to manipulate PDE2A expression, followed 48 h later by LPS (100 ng/ml) exposure for an additional 8 h.

Figure 2.

(A) The effect of Ad.PDE2A-shRNA versus the control Ad.Neg-shRNA. (B) The effect of PDE2A overexpression with Ad.PDE2A. PDE2A has two bands, as the exogenous PDE2A is coupled with GFP. The PDE2A and iNOS protein levels are normalized to GAPDH loading control and then to control MH-S cells exposed to LPS but not Ad (n=3 independent experiments). Values are means ± se. #P = 0.05 versus Ad.Neg-shRNA by ANOVA interaction; *P < 0.03 versus Ad.Neg-shRNA by ANOVA interaction.