Figure 6. Effect of 8-pCPT-cGMP and 8-CPT-cAMP on MH-S macrophage PDE2A and iNOS expression by Western blot with densitometric analysis measured after 8 h of exposure to LPS (100 ng/ml).

The PDE2A and iNOS protein levels are normalized to GAPDH loading control and then to control cells not exposed to LPS or cyclic nucleotide analog (n=3 independent experiments). Values are means ± se. *P < 0.005 versus 0 cyclic nucleotide value within each treatment group; #P < 0.002 versus 8-pCPT-cGMP by ANOVA interaction.