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. 2014 Nov;96(5):907–915. doi: 10.1189/jlb.3A0314-152R

Figure 7. Effect of down- or up-regulating PDE2A expression on mouse AM PDE2A and iNOS expression by Western blot with densitometric analysis of iNOS expression.

Figure 7.

AMs were obtained by lavage 6 days after pharyngeal-LPS (2.5 mg/g body weight) and 3 days after IT installation of Ad.PDE2A-shRNA, Ad.PDE2A, or water diluent. The isolated AMs were exposed to an additional 18 h of LPS (100 ng/ml) in vitro before processing. (A) Mouse AM homogenates from four mice were combined to generate the PDE2A and B-actin blots. (B) iNOS and B-actin blots are representative blots from a single mouse with densitometric analysis (n=4 mice). Values are means ± se. *P < 0.0001 versus all other groups.