Fig. 4.

WBP11 knockdown resembles and enhances PQBP1 knockdown phenotypes. (A) WBP11 MO (blue arrow) targets the 5′ UTR of both X. laevis wbp11 homeologs. (B) Expression of endogenous WBP11 was blocked by injection of 40 ng wbp11 MO (WB MO) but not control MO (CT MO). Endogenous (red arrowhead) and overexpressed (black arrowhead) WBP11 were detected by western blot with anti-WBP11 antibodies. An asterisk indicates a non-specific band that, along with β-tubulin staining, controls for sample loading. (C) Tailbud stage embryos injected into two dorsal cells at the four-cell stage with 100 ng control MO (CT), a mix of pqbp1 MOa and MOb (50 ng each; PQ MO) or 50 ng wbp11 MO (WB MO). (D) Neurula (stage 20, anterior view) embryos injected dorsally at the four-cell stage (schematic drawing) with 75 ng control MO (CT), 25 ng wbp11 MO (WB), a mixture of pqbp1 MOa plus MOb (25 ng each; 50 ng total; PQ) or a combination of wbp11 and pqbp1 MOa and MOb (25 ng each; 75 ng total; PQ+WB).