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. 2014 Oct;141(19):3697–3708. doi: 10.1242/dev.110833

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© 2014. Published by The Company of Biologists Ltd

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Fig. 5. — The thymus and parathyroids are absent at E17.5 in the Hoxa3^−/− mutants and after combined tissue-specific deletion, but present after tissue-specific deletion. H&E staining of transverse sections at E17.5 of Hoxa3^+/+ (A,F), Hoxa3^−/− (B,G), NCC deletion (Hoxa3^fx/−;Wnt1Cre^tg/0) (n=3) (C,H), endoderm deletion (Hoxa3^fx/−;Foxa2^CreERT2/+) (n=7) (D), and combined endoderm and NCC deletion (Hoxa3^fx/−;Foxa2^CreERT2/+;Wnt1Cre^tg/0) (n=2) (E,J) embryos. (I) An E17.5 transverse section that had been stained with DAPI and imaged for Gcm2-EGFP expression (Hoxa3^fx/−;Foxa2^CreERT2/+;Gcm2-EGFP) (n=2). (J) We were unable to identify parathyroids in the combined deletion mutants. In all sections, dorsal is towards the top of the image. thy, thymus; pt, parathyroid; *, missing organ. Scale bars: 80 µm.