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. 2014 Aug;141(15):2984–2992. doi: 10.1242/dev.109827

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Slmb is required for oocyte polarisation. (A,B) Kinesin-β-galactosidase (Kin-β-gal) visualised with an antibody against β-gal. Kin-β-gal accumulates at the posterior in wild-type (A) but is diffusely localised to the centre of slmb^9H4-17 germline clone oocytes (B). (C,D) Microtubule organisation in wild-type (C) and slmb^9H4-17 (D) stage 9 egg chambers visualised with Tau-GFP. (E,F) Egg chambers expressing matα4:GFP-Par-1N1S. GFP-Par-1 forms a posterior crescent in wild-type oocytes (E) but fails to localise to the posterior of most slmb^9H4-17 germline clone oocytes (F). (G,H) Par-1-GFP (expressed from a protein trap insertion in the endogenous par-1 locus) forms a posterior crescent in wild-type oocytes (G) but is usually absent from the posterior of hs-Slmb, slmb⁸/slmb^9H4-17 oocytes (H).