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. 2014 Aug;141(15):2984–2992. doi: 10.1242/dev.109827

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Slmb regulates follicle cell organisation and polarity. (A) The follicular epithelium is disrupted in hs-Slmb, slmb⁸/slmb^9H4-17 egg chambers 5 days after the last period of Slmb expression. Multiple layers of epithelial cells form at the posterior and anterior of mutant egg chambers. DAPI (blue) marks the nuclei and actin (green) reveals overall morphology. (B) A wild-type egg chamber showing the localisation of aPKC at the apical side of the follicle cells, which form a uniform monolayer around the oocyte. (C) Magnification of the posterior end of an hs-Slmb, slmb⁸/slmb^9H4-17 egg chamber 5 days after the last period of Slmb expression. aPKC remains apical in the cells that contact the germline, but is distributed in the cytoplasm and along the cortex of the unpolarised cells that no longer contact the germline. (D) A large slmb^9H4-17 clone forming a double-layered epithelium (outlined) stained with DAPI. (E-K) Follicle cell clones of slmb^9H4-17 (E-G,I-K) and slmb^8A6-5 (H) marked by the absence of GFP (green) or RFP (red in I), stained in red for aPKC (E,F,H), Crb (G), Baz (J) and Dlg (K), and in green for Par-6-GFP expressed from a genomic rescue construct (I). Cells in small mutant clones have reduced basal domains with higher levels of aPKC, Par-6 and Crb apically, but show normal localisation of Baz and Dlg. (F) A large slmb clone with a disrupted epithelial organisation and aPKC around the cortex. (L) Quantification of the phenotypes of slmb^9H4-17 follicle cell clones. Clones of fewer than 10 cells were scored as small. (M) Removing one copy of aPKC partially rescues the slmb mutant phenotype. Quantification of the percentage of stage 8, 9 and 10 egg chambers with an intact epithelial monolayer in CyO/+; hs-Slmb, slmb⁸/slmb^9H4-17 and aPKC^K06403/+; hs-Slmb, slmb⁸/slmb^9H4-17, dissected 4 days after hs-Slmb induction.