Fig. 1.

RAREs near Fgf8 and Hoxb1 bind RARs in mouse embryos. (A) Mouse Fgf8 RARE and alignment of sequences showing a highly conserved Fgf8 RARE; consensus RARE sequence is shown (Balmer and Blomhoff, 2005). (B) Hoxb1 gene harboring a repressive 5′-RARE and an activating 3′-RARE. ChIP was performed using chromatin from pooled E8.25 WT whole mouse embryos and antibodies against RAR isoforms or IgG (control); input DNA (diluted 100-fold) and immunoprecipitated DNA were analyzed by PCR using primers for the RAREs or for nonspecific regions (NSR) indicated by arrows. IP, immunoprecipitation; M, molecular size markers.