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. 2014 Aug;141(15):2972–2977. doi: 10.1242/dev.112367

Fig. 2.

Fig. 2.

RA directly represses caudal Fgf8 transcription through an upstream RARE. (A) BAC constructs used for generating Fgf8-lacZ and Fgf8-ΔRARE-lacZ transgenic embryos. Fgf8-ΔRARE-lacZ lacks the Fgf8 RARE, which was replaced by the 34 bp FRT-F3 site. (B) Transgenic 9-somite embryos carrying Fgf8-lacZ recapitulate endogenous caudal Fgf8 expression, whereas Fgf8-ΔRARE-lacZ exhibits ectopic expression in the three somites most recently generated (arrowheads, arrows) and presomitic mesoderm (psm; brackets). Left panels, dorsal view; right panels, lateral view. Scale bar: 100 µm.