Fig. 9.

Pharmacological rescue of SHF defects in conditional Nkx2-5 mutants. Pregnant females were treated with intraperitoneal injections of LiCl or NaCl from E8-E10 of gestation. (A-C) All Nkx2-5 conditional mutants treated with NaCl had a single OFT (PTA) at E17.5 (n=12). (D-G) 67% (n=10/15; P=0.01) of the conditional Rspo3 mutants (E-G) treated with LiCl had a significantly larger RV (arrows) and showed OFT septation. Treatment with LiCl did not correct the OFT alignment defects in the mutants (E). In A,D,E, aorta and pulmonary artery are indicated by arrows. Ao, aorta; LV, left ventricle; OFT, outflow tract; Pa, pulmonary artery; PTA, persistent truncus arteriosus; RV, right ventricle.