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. 2014 Oct 14;55(10):6398–6408. doi: 10.1167/iovs.14-14623

Figure 1.

Figure 1

Graphical representation of deamidated αA crystallin transgenic construct used for generation of CRYAAN101D transgenic mice. Reprinted with permission from Gupta R, Asomugha CO, Srivastava OP. The common modification in alphaA-crystallin in the lens, N101D, is associated with increased opacity in a mouse model. J Biol Chem. 2011;286:11579–11592. Copyright 2011 American Society for Biochemistry and Molecular Biology. Transgene construct contain αA crystallin promoter, human β-globin intervening sequence, the coding region of αA crystallin that has deamidation at 101 position, with an in-frame epitope tag (7X-His) at the N-terminus of αA crystallin and untranslated sequences (UTT) and polyadenylatation SV40 sequence at the C-terminus.