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. 2014 Oct 14;55(10):6398–6408. doi: 10.1167/iovs.14-14623

Figure 5.

Figure 5

Expression of Na,K-ATPase is downregulated in N101D lenses. (A) Immunofluorescent staining of α-subnit of Na,K-ATPase (red) in 1-month-old N101D lenses (b) was drastically reduced compared with 1-month-old WT lenses (a). No significant difference in immumostaining of Na,K-ATPase in 5-month-old WT (c) and N101D lenses (d) was observed. Nuclei were counterstained with DAPI. Scale bar: 60 μm. (B) Western blot analysis confirming the decreased expression of Na, K-ATPase in 1-month-old N101D compared with 1-month-old WT lenses. Lens homogenates from 1-month-old N101D and 1-month-old WT mice (n = 2) were used for the immunoblot. β-actin was used as a loading control.