Fig. 6.

Patterning of lingual papillae is partially disrupted in the anterior of the Fz3^CKO/−;Fz6^−/−;K14-Cre tongue. (A-D) Dorsal views of a Fz3^CKO/+;Fz6^−/−;K14-Cre;K17-GFP tongue (A,B) and a Fz3^CKO/−;Fz6^−/−;K14-Cre;K17-GFP tongue (C,D) at P20 showing the locations of taste buds (A,C) and epithelial morphology with AM4-65 and GFP fluorescence (B,D). The pattern in B matches that of wild-type tongues. Anterior is downwards. The rosette pattern is present along the midline at ∼70% of the distance from the tip to the base of the tongue. The grayscale images on the right (panels a-f) correspond to the locations of the red squares in the low-magnification images on the left. The orientation of each papilla in a-f was scored with an arrowhead or, in cases where the polarity of the papillae is ambiguous, with an oval. Scale bar: 1 mm. (E) Cross-sections of the anterior dorsal surface of a control Fz3^CKO/+;Fz6^−/−;K14-Cre;K17-GFP tongue (left; wild-type pattern) and a Fz3^CKO/−;Fz6^−/−;K14-Cre;K17-GFP tongue (right) at 3 months of age. Arrowheads indicate some of the mis-oriented papillae in the right panel. The mice were injected intraperitoneally with AM4-65 1 day prior to sacrifice. A, anterior; P, posterior. Scale bar: 500 μm.