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Nkx3.2 and GATA genes display mutually exclusive expression patterns in vitro and in vivo. (A) Chicken presomitic mesoderm (PSM) was cultured in the presence of SHH and BMP4 for the indicated time period, and gene expression was assayed by RT-PCR (n=4). (B-D) WISH analysis of HH stage 12-13 chicken embryos. (E-G) Sections of embryos in B-D. (H) WISH analysis for Nkx3.2 and Gata4 in HH stage 30 chicken embryos. s, somite.
