Fig. 4.

grk mRNA is associated with polysomes and large RNP complexes. (A-C′) UV absorbance profile of fractionated ovary extracts with genotypes indicated, with (right) and without (left) EDTA treatment. All profiles were adjusted to the same scale with the maximum UV absorbance indicated by the double arrowheads and the baseline indicated by the single arrowheads. Lack of UV absorbance in the fast-sedimenting fractions of the EDTA-treated gradients suggests that the addition of EDTA completely disrupted the polysomes. (D) Distribution of grk (green triangles), αTub67C (blue circles) and RpL32 (red squares) mRNA in the wild-type gradient were analyzed by qRT-PCR. Changes in mRNA distribution are evident between D (the wild-type gradient) and D′ (the EDTA-treated wild-type gradient). For each mRNA, data are plotted as the percentage of total amount applied to the gradient detected in each fraction; error bars represent standard error from four (D) or three (D′) independent experiments.