Fig. 4.

zyx-1 mutant synapses retract during development. (A) Timecourse of PLM synapse structure of representative animals. Individual wild-type or zyx-1(gk190) animals were immobilized in 10 mM sodium azide at 12 hph, imaged at 100 ×, recovered on an NGM plate, and then reimaged in similar fashion at 24 hph and 48 hph. The zyx-1 examples illustrate that PLM synapse loss is stochastic. In the top example, one synapse is lost while the other grows. Furthermore, they illustrate that PLM synapses can be lost both early (bottom) as well as late (top) in development. Scale bar: 10 µm. (B) Temperature shift of zyx-1(gk190) animals showing the PLM synapses in L4 larvae as a function of the time of shift (from permissive to non-permissive temperature, or vice versa). n=127-253 animals for shifted time points and n=95-173 for unshifted controls. Strains used were NM3361 and NM3413.