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. 2014 Oct;141(20):3922–3933. doi: 10.1242/dev.108217

Fig. 5.

Fig. 5.

ZYX-1 functions cell-autonomously to regulate mechanosensory synapse development. (A) The expression of full-length ZYX-1A cDNA in presynaptic mechanosensory neurons (Pmec-7::mCherry::zyx-1::GFP) rescues the zyx-1 mutant phenotype, whereas muscular or interneuronal expression does not. PLM synapses formed per animal are displayed for wild type, zyx-1, and zyx-1 mutants expressing zyx-1::GFP fusions under the control of distinct promoters. Also shown are rescues by a complete zyx-1 genomic construct and a genomic construct only capable of expressing zyx-1b from the P4 promoter. (B) The expression of ZYX-1 C-terminal LIM domains in mechanosensory neurons rescues the PLM synapse defects of zyx-1 mutants. PLM synapses formed per animal are displayed for wild type, zyx-1, and zyx-1 mutants expressing various domains of ZYX-1 under the control of the mec-7 promoter. (A,B) Error bars indicate mean ± s.e.m. n=58-897. (C) Subcellular localization of ZYX-1 LIM domain fusions. The structure of various ZYX-1 domain constructs is depicted alongside the localization pattern observed in PLM and PVM, and the phenotypic consequences of expression of the construct in touch receptor neurons. Many LIM domain constructs were able to traffic to the nucleus, but the rescuing activity of the constructs was independent of subcellular localization. Scale bar: 10 μm.