Figure 6.

Chemical coding of transduced myenteric cells in the colon after systemic scAAV8 injection. Similarly to scAAV9, systemic injection of scAAV8-CB-GFP produced GFP expression (B,E,H,K,N,Q) was detected in ChAT (A–C), calbindin (D–F), and calretinin (G–I) positive excitatory neurons, interneurons and intraganglionic fibers. Additionally, GFP expression was also detected in VIP (J–L) and nNOS (M–O) positive inhibitory neurons, interneurons, and intraganglionic fibers. GFP expression was occasionally detected in S100 positive enteric glia (P–R). White arrows in merged images indicate transduced cells. Scale bars are 100 μm.