Table 3.
Oligonucleotides used in the study.
| Gene (Gene symbol) | Primer 5′ → 3′ (sense, antisense) | Genbank no. | TAn (°C) | Product-length (bp) | References |
|---|---|---|---|---|---|
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | accacagtccatgccatcac | NM_002046 | 59 | 452 | Metzger et al., 2007 |
| tccaccaccctgttgctgta | |||||
| Hypoxanthine phosphoribosyl-transferase 1 (HPRT) | tgaacgtcttgctcgagatgtg | NM_000194.2 | 60 | 125 | Bottner et al., 2010 |
| ccagcaggtcagcaaagaattt | |||||
| Mitochondrial ribosomal protein L15 (MRPL15) | Unknown | NM_014175 | 60 | 125 | QuantiTect Primer Assay 200; Qiagen |
| Neurotrophin receptor p75, (NGFR) | tgagtgctgcaaagcctgcaa | NM_002507 | 60 | 230 | Tsunoda et al., 2006 |
| tctcatcctggtagtagccgt | |||||
| Ret receptor (RET) | agatttcggatttcggcttgt | NM_020630.4 | 63 | 161 | Metzger et al., 2009 |
| ccacagcaggacaccaaaaga | NM_020975.4 | ||||
| NM_020630.4 | |||||
| NM_020975.4 | |||||
| Cholinaceyl transferase (CHAT) | tggtgcaatcagttctttgt | NM_020549.3 | 55 | 239 | Designed by using NCBI/Primer-BLAST |
| aggcagatgcagcgctcaatcatgtc | NM_020984.2 | ||||
| NM_020986.2 | |||||
| NM_020985.2 | |||||
| Nitric oxide synthase (NOS1) | tccctcctcgggcttctcgc | NM_000620.2 | 60 | 193 | Designed by using NCBI/Primer-BLAST |
| cccacagcgacggccatgtt | |||||
| Tyrosine hydroxylase (TH) | acggtggagttcgggctgtg | NM_199293.2 | 60 | 248 | Designed by using NCBI/Primer-BLAST |
| aaggggcgctggatgcgtg | NM_199292.2 | ||||
| NM_000360.3 | |||||
| S100 calcium binding protein B (S100B) | aggacccgcagcagagacga | NM_006272.2 | 60 | 102 | Designed by using NCBI/Primer-BLAST |
| tcgatgagggccaccatggc | |||||
| Tropomyosin 1 (alpha) (TPM) | ctcgcagaaggaagacagatatgag | NM_001018020 | 60 | 101 | Bottner et al., 2010 |
| tagttactgacctctccgcaaactc | |||||
| α-Synuclein (SNCA) | tcacgccttgccttcaagccttct | NM_000345.3 | 60 | 148 | Designed by using NCBI/Primer-BLAST |
| ccacaactccctccttggcctt | |||||
| Caspase 3 (CASP3) | cctgctcacactcggcgctc | NM_004346.3 | 60 | 172 | Designed by using NCBI/Primer-BLAST |
| tccagagtccattgattcgcttcca | |||||
| Catalase (CAT) | gcccgatgtgcatgcaggaca | NM_001752.3 | 60 | 167 | Designed by using NCBI/Primer-BLAST |
| tgcccgcacctgagtaacgt | |||||
| Superoxide dismutase 2 (SOD2) | aggctcaggttggggttggct | NM_000636.2 | 60 | 135 | Designed by using NCBI/Primer-BLAST |
| gcgtgctcccacacatcaatccc | |||||
| Telomerase reverse transcriptase (TERT) | gctgctcaggtctttcttttatgtc | NM_198253.2 | 60 | 116 | Designed by using NCBI/Primer-BLAST |
| tcaagtgctgtctgattccaatg | |||||
| Nav1.1 (SCN1A) | gaagaacagcccgtagtggaa | NM_006920.4 | 60 | 225 | Candenas et al., 2006 |
| ttcaaatgccagagcacca | NM_006920.4 | ||||
| Nav1.2 (SCN2A) | gaaggcaaagggaaactctgg | NM_001040143.1 | 60 | 297 | Candenas et al., 2006 |
| cagtgagacatcaacaatcaggaag | NM_001040142.1 | ||||
| NM_021007.2 | |||||
| Nav1.3 (SCN3A) | aaaccccaactatggctacacaa | NM_001081677.1 | 60 | 367 | Candenas et al., 2006 |
| tcctaacccacctattccactga | NM_006922.3 | ||||
| NM_001081676.1 | |||||
| Nav1.4 (SCN4A) | caacaacccctacctgaccatac | NM_000334.4 | 60 | 317 | Candenas et al., 2006 |
| gcagagtccaccacttcttcc | |||||
| Nav1.5 (SCN5A) | ccgccatttacacctttgagt | NM_001099405.1 | 60 | 294 | Candenas et al., 2006 |
| cgctgaggcagaagactgtg | NM_001099404.1 | ||||
| NM_000335.4 | |||||
| NM_198056.2 | |||||
| Nav1.6 (SCN6A) | aaggttgtgtccagcggttc | NM_014191.2 | 60 | 207 | Candenas et al., 2006 |
| ggatggtgcggatggtctt | |||||
| Nav1.7 (SCN9A) | cccacagaccccaggagcga | NM_002977.2 | 60 | 180 | Designed by using NCBI/Primer-BLAST |
| tggtcgtgccctctggcaga | |||||
| Nav1.8 (SCN10A) | tggaattccccattggatccctcg | NM_006514.2 | 60 | 175 | Designed by using NCBI/Primer-BLAST |
| gctttcaagtccagctggggcc | |||||
| Nav1.9 (SCN11A) | ggcaggctgttttattcccgcc | NM_014139.2 | 60 | 151 | Designed by using NCBI/Primer-BLAST |
| tgcagccagagagtcggaagtga |