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. 2014 Oct 15;4:144. doi: 10.3389/fcimb.2014.00144

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Copyright © 2014 Rathnaiah, Lamont, Harris, Fenton, Zinniel, Liu, Sotos, Feng, Livneh-Kol, Shpigel, Czuprynski, Sreevatsan and Barletta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–E) Southern blot analysis of MAP wild type K-10 and transposon insertion mutants. DNA samples from Tn5367 mutants and the wild type strain (K-10a) were digested with EcoRI (A,B,E). DNA samples from the Tn5370 mutant STM68 and the wild type strain (K-10b) were digested with NheI and NdeI (C,D). DNA samples were run on agarose gels, transferred to a nylon membrane and hybridized with the IS1096 probe to verify the presence of the transposon (A–C) and the IS900 probe to confirm that the mutants were derived from MAP (C,D). (B–E) are hybridization images generated from the same Southern transfer, while (A) was from an independent transfer.