Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Oct 15;4:144. doi: 10.3389/fcimb.2014.00144

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Rathnaiah, Lamont, Harris, Fenton, Zinniel, Liu, Sotos, Feng, Livneh-Kol, Shpigel, Czuprynski, Sreevatsan and Barletta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Analysis of MAP Tn5367 (3,381 bp) and Tn5370 (2295 bp) insertion mutants by PCR. Lane 1, Invitrogen TrackIt™ 1 Kb Plus DNA Ladder; Lanes 2 and 9, empty. Amplicons for the DNA template-primer combinations and corresponding sizes are: Lanes 3 and 7, K-10 with MAP1566-F and MAP1566-R, 1179 bp; Lane 4, STM68 with MAP1566-F and MAP1566-R, 3474 bp; Lane 5, K-10 with 2E11-IP-F and 2E11-IP-R, 1845 bp; Lane 6, 2E11 with 2E11-IP-F and 2E11-IP-R, 5226 bp; Lane 8, 30H9 MAP1566-F and MAP1566-R, 4560 bp; Lane 10, STM68 with MAP1566-F and AMT32, 1160 bp; Lane 11, 2E11 with 2E11-IP-F and AMT32, 1890 bp; Lane 12, 3H4 with 3H4-R and AMT32, 1974 bp; Lane 13, 4H2 with 4H2-F and AMT32, 1431 bp; Lane 14, 22F4 with 22F4-F and AMT32, 561 bp; Lane 15, 30H9 with MAP1566-R and AMT32, 941 bp. Relevant band sizes (bp) for the DNA ladder are displayed to the left.