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. Author manuscript; available in PMC: 2015 Apr 14.
Published in final edited form as: Nat Commun. 2014 Oct 14;5:5180. doi: 10.1038/ncomms6180

Figure 4.

Figure 4

Localization of early endosomes was altered in the axons of developing strip projection neurons (PNs)

(a–f) A GFP-fused FYVE domain (GFP-FYVE) was expressed in PNs, and its localization was investigated at 21 h after puparium formation (APF). Axons of wild-type (WT, a–c) and stripdogi PNs (d–f). (a, d) GFP-FYVE and MARCM neuroblast clones labelled by mCD8-RFP are shown in green and magenta, respectively. (b,e) Only green channels are shown in grey scale. (c) and (f) are magnified images of dotted rectangles of (b) and (e), respectively. Scale bar: 25 μm. (g) The intensity of GFP-FYVE in the axon stalk (200 μm2) was normalized to the intensity of mCD8RFP and then plotted. Error bars are defined as SEM. **p = 0.0047, t-test.