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. 2014 Aug 1;6(9):1191–1204. doi: 10.15252/emmm.201303283

Figure 2. Poly(I:C) and anti-CD40 Ab allow generation of memory CD8+ T cells after Langerin targeting.

Figure 2

CD8+ T cells purified from [OT-I × Ly5.1] F1 mice were labeled with CFSE and injected i.v. into C57BL/6 mice. The next day, mice were immunized i.d. into both ears with 0.5 μg Langerin/OVA (L31) alone or in addition to imiquimod (+imiq) or poly(I:C) and anti-CD40 (+pIC/40). Data from individually analyzed mice are pooled from three independent experiments and compared using one-way ANOVA followed by Tukey's test (n.s.: non-significant, P > 0.05).

  1. Six days or 8 weeks after immunization, the proportions (L31: six mice; L31+imiq: nine mice; L31+pIC/40: five mice; ANOVA: P = 0.0002 at day 6, P = 0.0001 at week 8) and absolute numbers (L31: four mice; L31+imiq: five mice; L31+pIC/40: five mice; ANOVA: P = 0.0011 at day 6, P = 0.0061 at week 8) of CD45.1+ CD8+ T cells in skin-draining lymph nodes were evaluated.
  2. After 8 weeks, total lymph node cells were exposed overnight to the OVA peptide SIINFEKL. CD62L expression and IFN-γ production were visualized in CD45.1+ CD8+ T cells by flow cytometry. Representative stainings.
  3. Percentage of CD62L-low IFN-γ-producing among OT-I CD8+ T cells (L31: four mice; L31+imiq: five mice; L31+pIC/40: five mice; ANOVA: P = 0.0024).