Figure 4. The TIM23 complex facilitates import of Om45.

A–D The indicated ³⁵S-labeled proteins were imported into tim23-71,78, tim50-279,282,286, or corresponding wild-type mitochondria and analyzed as in Fig 3B–D.

E–G The indicated ³⁵S-labeled proteins were imported into mitochondria with (NONE) or without (Val. or CCCP) ∆Ψ at 25°C and analyzed as in (A–D).

H The indicated ³⁵S-labeled proteins were imported into mitochondria for 10 min at 25°C. Then, mitochondria (−Swelling) were converted to mitoplasts (+Swelling) and treated with 50 μg/ml PK. Proteins were analyzed as in (A–D).

I ³⁵S-labeled Om45-100 was imported into mitochondria isolated from tim23-71,78, tim50-279,282,286, and their corresponding wild-type cells (TIM23 and TIM50) at 25°C and analyzed as in (A–D).