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. 2014 Sep 1;28(17):1885–1899. doi: 10.1101/gad.246819.114

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© 2014 Sexton et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — Intragenic complementation resolves multiple TPP1 roles in telomere length maintenance. (A) Schematic of TPP1 variants used for overexpression at the AAVS1 locus. (B) Telomerase primer extension activity of telomerase purified by the indicated TPP1-F after overexpression in HEK293T cells. Relative activity is normalized for background and the recovery control (RC). (C) IF of TPP1-F variants expressed from AAVS1 in the TPP1 ΔL-GFP background stained against Flag and TRF1, with AAVS1-expressed GFP cells as a negative control for the Flag antibody background. (D) Telomere length of TPP1 ΔL/ΔL cell lines expressing TPP1 variants. Note that the same TPP1-F sample is used here and in Figure 5D.