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. 2014 Aug 15;28(16):1772–1785. doi: 10.1101/gad.245738.114

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© 2014 Hayashi et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0.

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Figure 4. — A piwi transgene lacking int4 rescues transposon derepression in EJC-depleted cells. (A) Expression of wild-type GFP-piwi, but not of GFP-piwi Δint4, is dependent on EJC factors. Shown are confocal sections through egg chambers expressing GFP-piwi or GFP-piwi Δint4 depleted for the indicated genes in germline cells. Bars, 10 μm. Relative GFP levels (±standard deviation) from the indicated GFP-piwi transgenes in comparison with the white control knockdown are indicated (GFP fluorescence levels in nurse cell nuclei normalized to those in follicle cell nuclei from three egg chambers). (B) Displayed are fold changes in steady-state RNA levels of HeT-A and blood in ovaries depleted for the indicated genes in the germline and expressing the indicated GFP-piwi transgenes. RNA levels are normalized to rp49 levels, and averages of three biological replicates relative to control knockdowns are shown. Error bars indicate standard deviation. (*) P < 0.01; (**) P < 0.001. TE derepression caused by Acn knockdown is rescued by GFP-piwi Δint4 but not by wild-type GFP-piwi. (C) Shown are the X-gal stainings of egg chambers depleted for the indicated genes in somatic follicle cells and expressing the gypsy-lacZ reporter and the indicated GFP-piwi transgenes. GFP-piwi lacking intron4, but not wild-type GFP-piwi, greatly reduces the gypsy-lacZ expression induced by the depletion of Acn.