Skip to main content
. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Neuron. 2014 Oct 1;84(1):107–122. doi: 10.1016/j.neuron.2014.09.012

Figure 8. NMDA receptor-deficient PV neurons have reduced presynaptic networks.

Figure 8

(A) Simultaneous TMP-inducible Grin1 cKO and expression of two fluorescent reporters in PV neurons.

(B–F) Newborn Pvalb:2A-DD-Cre/Ai9/Grin1flox/flox and Pvalb:2A-DD-Cre/Ai9/Grin1wt/wt mice were injected into the cerebral lateral ventricles with AAVDJ DIO-SyP-GFP. Sparse recombination was triggered with a single TMP pulse at P9 (0.3 mg/gm, as depicted in panel A). Morphologies of PV neurons in the DG were analyzed at P25.

(B) Images of PV cells expressing tdTomato and synaptophysin-GFP. Dashed lines mark the GCL (based on DAPI staining, not displayed).

(C) Images of presynaptic boutons formed by single axon segments in the GCL.

(D) Total densities of axonal/presynaptic puncta in the GCL, linear puncta density per axon branch, and puncta diameters. Total density: Control, N=10 mice/24 neurons; cKO, N=4 mice/12 neurons, P=0.001. Linear density and diameters: N=2 mice/7–9 neurons/genotype, P=0.53 and 0.12, respectively.

(E) Examples of reconstructed dendritic trees.

(F) Analysis of dendrite morphologies. Control, N=8 mice/24 neurons; cKO, N=4 mice/12 neurons. Trees, P=0.92; Nodes, P=0.49; Dendrites, P=0.27; Segments, P=0.46.

See also Table S2.