Figure 6.

Enhanced differentiation of erythroblast into reticulocyte by UF. After 2 days in culture, cells were conjugated with anti-TER119 magnetic particles, and TER119⁺ cells were isolated using a magnet. A, Reticulocytes were characterized based on their being enucleated. DRAQ5 dye was used to distinguish nucleated and nonnucleated cells. B, May-Grünewald staining of TER119⁺ cells. Data are presented as mean ± SEM; n = 4. Means with different superscript letters are significantly different (P < .05).