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. 2014 Aug 22;155(11):4433–4446. doi: 10.1210/en.2014-1304

Figure 6.

Figure 6.

L148S triggers ERK1/2 phosphorylation in a β-arrestin–dependent manner. A and B, A representative autoradiograph (A) and densitometric analysis (B) showing the expression of total and phosphorylated ERK1/2 in YFP-L148S–overexpressing WT and β-arrestin-1 and -2 double-KO (dKO) MEFs after 0- and 10-minute Kp-10 treatment (100nM). Western blot analyses were done using monoclonal anti-ERK1/2 and anti-phospho–ERK1/2 antibodies. The data represent the mean of 3 independent experiments. *, P < .05, pERK1/2/total ERK1/2 ratio of Kp-10–stimulated L148S-expressing WT MEFs compared with unstimulated L148S-expressing WT MEFs. Error bars represent SEM.