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A, representative overlay of flow cytometry histograms of HEK293 cells expressing Kir2.1 with GFP or with Cav-1-YFP. The cells were stained with primary HA antibody and Alexa Fluor 555 secondary antibody. B, expression of Kir2.1 co-expressed with Cav-1 normalized to Kir2.1 co-expressed with GFP from flow cytometry data (n = 3). Cav, caveolin; HA, haemagglutinin.
