Figure 5. Effects of 5HMF on phosphatidylserine (PS) exposure in red blood cells (RBCs) from patients with sickle cell disease (SCD).

After a 60 min pre-incubation, RBC suspensions in Cl-MBS were deoxygenated (20 mmHg O₂) in shaken 24-well plates in a low oxygen incubator for up to 4 h in the absence and presence of 5HMF (5 mm). Aliquots were removed at intervals and the percentage of RBCs positive for PS exposure measured using FACS. Data are means ± s.e.m., n = 4. #P < 0.05 for control RBCs at time 0 versus at 240 min. *P < 0.05 for control RBCs in the absence of 5HMF versus those incubated in its presence at 240 min.