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. 2014 May 5;33(11):1256–1270. doi: 10.1002/embj.201387330

Figure 3. STAG3 and synapsis-related proteins.

Figure 3

  1. Immunofluorescence staining of spermatocyte chromosome spreads of wt and Stag3ko/ko mice, probed with anti-SYCP3 for axial elements (AEs) and anti-SYCP1 for synapsed axes; nucleic acids were stained with DAPI. Four examples of Stag3ko/ko nuclei are shown, representing different levels of SYCP1 staining. Five SYCP3/SYCP1 structures are shown magnified at the bottom of the figure and are numbered.
  2. Immunofluorescence staining of spermatocyte chromosome spreads of wt and Stag3ko/ko mice, probed with anti-SYCP3 for AEs and anti-HORMAD1 for unsynapsed axes; nucleic acids were stained with DAPI. Three examples of Stag3ko/ko spreads are shown, displaying different levels of HORMAD1 staining, including occasional aggregates and local accumulation.
  3. Immunofluorescence staining of spermatocyte chromosome spreads of wt and Stag3ko/ko mice, probed with anti-SYCP3 for AEs and anti-DMC1 for double-strand break repair foci.

Data information: nucleic acids were stained with DAPI. Size bars indicate 10 μm.