Figure 4. Centromeres and telomeres in Stag3^ko/ko spermatocytes.

1. Immunofluorescence staining of spermatocyte chromosome spreads of wt and Stag3^ko/ko mice, probed with anti-SYCP3 and anti-centromere antibody (ACA); nucleic acids were stained with DAPI. Three examples of Stag3^ko/ko spreads are shown to indicate centromere cluster formation and to highlight partial co-localization of SYCP3 with centromeres and the structures of these regions. Three areas are provided as magnified excerpts. Size bars indicate 10 μm.
2. Immunofluorescence staining of spermatocyte chromosome spreads of wt and Stag3^ko/ko mice, probed with anti-SYCP3 and anti-RAP1 to stain telomeres; nucleic acids were stained with DAPI. Three examples of Stag3^ko/ko spreads are shown to represent different stages and to show an example of telomere cluster formation, which is shown in a magnified excerpt as well. Size bars indicate 10 μm.